Skip to main content
 

A Charged Situation

Capillary Zone Electrophoresis and Drug Screening

By Nora S. Green

A Charged Situation


 

Abstract

In this interrupted case, students take on the role of a trainee forensic chemist completing their final performance test in a toxicology laboratory. The trainee’s performance test involves a healthy man who was found dead in his apartment. Numerous pill bottles around the apartment lead investigators to suspect that one or more of the pharmaceutical compounds could have contributed to his death. In the role of the trainee, students must consider two different liquid-liquid extraction methods and two different capillary zone electrophoresis methods to determine if any of the pharmaceuticals are found in the victim’s blood. The case was developed for an upper-level forensic chemistry course as part of a section on acid/base chemistry, extractions, and separation techniques used in forensic chemistry. The case could also be used in an analytical or instrumental analysis course.

   

Date Posted

05/28/2025

Overview

Objectives

  • Identify acidic and basic functional groups in drug molecules.
  • Use the relationship between pH and pKa to predict the ionization state of a drug.
  • Explain the principles of liquid-liquid extraction.
  • Explain the principle of separation in capillary zone electrophoresis.
  • Predict order of separation of molecules in capillary zone electrophoresis.

Keywords

Forensic chemistry; analytical chemistry; electrophoresis; drugs; drug screening; acid/base; liquid extraction; toxicology; capillary zone electrophoresis; CZE

  

Subject Headings

Analytical Chemistry
Chemistry (General)
Forensic Science

EDUCATIONAL LEVEL

Undergraduate upper division

  

FORMAT

PDF

   

TOPICAL AREAS

N/A

   

LANGUAGE

English

   

TYPE/METHODS

Interrupted

 

 

Asset 2