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When Hypotheses Are Unsupported

Investigating a Vanishing Fish Population

By Krystal A. Nunes, Najwa Abadi, Alexander Gopaul

When Hypotheses Are Unsupported


 

Abstract

This interrupted case study is designed to highlight the importance of “failure” as part of the scientific process. Hypotheses that are disproven or experiments that yield unexpected results may feel discouraging, but they often provide critical information that drives future discoveries. The case narrative presents a fictitious scenario involving the mysterious decline of a native fish population. Students are asked to analyze the scenario using only the information provided, formulate a hypothesis to explain the environmental concern, and design an experiment to test it. New information is then revealed that requires students to discard their initial hypothesis and experimental design, reassess the scenario, and propose a revised approach. The case concludes with the presentation of simple graphs for students to interpret, allowing them to evaluate evidence and test the strength of their conclusions. Originally developed for a first-year introductory biology course at the undergraduate university level, the case is designed to develop skills of hypothesis writing and experimental methodology creation, while also addressing themes related to conservation biology, invasive species, and population dynamics, all of which are aligned with first-year biology curricular topics.

   

Date Posted

09/21/2026

Overview

Objectives

  • Recognize how observations from an ecosystem can be used to generate scientific hypotheses.
  • Construct testable hypotheses that are based on observations and identify relationships between variables.
  • Design an experiment that appropriately controls variables and produces quantifiable data to test a scientific hypothesis.
  • Analyze experimental data to determine whether results support or refute a hypothesis.
  • Evaluate alternative explanations for ecological change using provided evidence.
  • Justify conclusions about ecological interactions (e.g., competition, invasive species dynamics) using sound scientific reasoning.

Keywords

Experimental design; hypothesis testing; scientific method; failure; invasive species; ecology

  

Subject Headings

Biology (General)
Ecology
Science (General)

EDUCATIONAL LEVEL

High school, Undergraduate lower division

  

FORMAT

PDF

   

TOPICAL AREAS

Scientific method

   

LANGUAGE

English

   

TYPE/METHODS

Interrupted

 

 

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