Although evidence exists that online education can result in comparable outcomes as the equivalent face-to-face (F2F) version, there is still a dearth in the literature. The objective of this pilot was to investigate differences in academic performance between students participating in the F2F versus online version of Nutrition 10, an introductory, college-level nutrition course. Students enrolled in Nutrition 10 (F2F) (n=907) and Nutrition 10V (virtual) (n=1,239) completed a 27-item nutrition knowledge questionnaire before (pre-) and after the class (post-) developed from the learning objectives (=0.92). Students that took the class, regardless of delivery method, improved nutrition knowledge (+6.9 points; p<0.01). In the F2F class, students exhibited a greater improvement in nutrition knowledge (+7.7 points; p<0.01) compared to the virtual class (+6.3 points; p<0.01). There were significant differences in grades based on the quarter the course was offered. Fall 2019 F2F students received a grade that was 2.9% greater than the virtual course (p<0.05), whereas Winter 2020 virtual students received a grade that was 1.2% greater than the F2F course (p<0.01). Although both F2F and online education share many similarities, there are still significant differences that remain between the two modalities.